PDBe 5thr

Electron Microscopy
8.9Å resolution

Cryo-EM structure of a BG505 Env-sCD4-17b-8ANC195 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 21-mer (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Envelope glycoprotein gp160 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 153 amino acids
Theoretical weight: 17.15 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: NEW Q2N0S6 (Residues: 509-661; Coverage: 18%)
Gene name: env
Envelope glycoprotein gp160 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 481 amino acids
Theoretical weight: 54.06 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: NEW Q2N0S6 (Residues: 30-505; Coverage: 55%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
T-cell surface glycoprotein CD4 Chains: G, H, I
Molecule details ›
Chains: G, H, I
Length: 192 amino acids
Theoretical weight: 21.47 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: NEW P01730 (Residues: 26-207; Coverage: 42%)
Gene name: CD4
Sequence domains: Immunoglobulin C2-set domain
17b Fab VL domain Chains: J, K, L
Molecule details ›
Chains: J, K, L
Length: 214 amino acids
Theoretical weight: 23.4 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
17b Fab VH domain Chains: M, N, O
Molecule details ›
Chains: M, N, O
Length: 229 amino acids
Theoretical weight: 24.46 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
8ANC195 G52K5 VH domain Chains: P, R, T
Molecule details ›
Chains: P, R, T
Length: 244 amino acids
Theoretical weight: 26.13 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
8ANC195 G52K5 VL domain Chains: Q, S, U
Molecule details ›
Chains: Q, S, U
Length: 215 amino acids
Theoretical weight: 23.4 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8.9Å
Relevant EMDB volumes: EMD-8407
Expression systems:
  • Homo sapiens
  • Spodoptera frugiperda