PDBe 5th9

X-ray diffraction
3Å resolution

Structure determination of a potent, selective antibody inhibitor of human MMP9 (GS-5745 bound to MMP-9)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
GS-5745 Fab heavy chain Chains: H, I, J
Molecule details ›
Chains: H, I, J
Length: 230 amino acids
Theoretical weight: 24.81 KDa
Source organism: Oryctolagus cuniculus
Expression system: Oryctolagus cuniculus
GS-5745 Fab light chain Chains: L, M, N
Molecule details ›
Chains: L, M, N
Length: 214 amino acids
Theoretical weight: 23.42 KDa
Source organism: Oryctolagus cuniculus
Expression system: Oryctolagus cuniculus
67 kDa matrix metalloproteinase-9 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 231 amino acids
Theoretical weight: 25.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14780 (Residues: 40-443; Coverage: 33%)
Gene names: CLG4B, MMP9

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P43212
Unit cell:
a: 168.167Å b: 168.167Å c: 234.735Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.218 0.257
Expression systems:
  • Oryctolagus cuniculus
  • Escherichia coli