PDBe 5tff

X-ray diffraction
1.89Å resolution

Nucleotide-binding domain 1 of the human cystic fibrosis transmembrane conductance regulator (CFTR) with UTP

Source organism: Homo sapiens
Entry authors: Wang C, Aleksandrov AA, Yang Z, Forouhar F, Proctor E, Kota P, An J, Kaplan A, Khazanov N, Boel G, Stockwell BR, Senderowitz H, Dokholyan NV, Riordan JR, Brouillette CG, Hunt JF

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Cystic fibrosis transmembrane conductance regulator Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 25.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Canonical: P13569 (Residues: 387-646; Coverage: 15%)
Gene names: ABCC7, CFTR
Sequence domains: ABC transporter

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P43
Unit cell:
a: 40.183Å b: 40.183Å c: 141.794Å
α: 90° β: 90° γ: 90°
R R work R free
0.162 0.16 0.19
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'