PDBe 5tdd

X-ray diffraction
1.55Å resolution

Human UBR-box from UBR2 in complex with HIFS peptide


Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase UBR2 Chain: A
Molecule details ›
Chain: A
Length: 76 amino acids
Theoretical weight: 8.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q8IWV8 (Residues: 98-168; Coverage: 4%)
Gene names: C6orf133, KIAA0349, UBR2
Sequence domains: Putative zinc finger in N-recognin (UBR box)
HIS-ILE-PHE-SER peptide Chain: B
Molecule details ›
Chain: B
Length: 4 amino acids
Theoretical weight: 504 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P41
Unit cell:
a: 29.313Å b: 29.313Å c: 74.151Å
α: 90° β: 90° γ: 90°
R R work R free
0.111 0.106 0.152
Expression systems:
  • Escherichia coli
  • Not provided