PDBe 5t2w

X-ray diffraction
2.2Å resolution

Structure of thymine DNA glycosylase bound to substrate analog 2'-F-5-formyl-dC

Released:

Function and Biology Details

Reaction catalysed:
Hydrolyzes mismatched double-stranded DNA and polynucleotides, releasing free thymine. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
G/T mismatch-specific thymine DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 25.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13569 (Residues: 82-308; Coverage: 55%)
Gene name: TDG
Sequence domains: Uracil DNA glycosylase superfamily
DNA (27-MER) Chain: D
Molecule details ›
Chain: D
Length: 28 nucleotides
Theoretical weight: 8.61 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (28-MER) Chain: C
Molecule details ›
Chain: C
Length: 28 nucleotides
Theoretical weight: 8.65 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C2
Unit cell:
a: 83.53Å b: 51.9Å c: 82.03Å
α: 90° β: 101.52° γ: 90°
R-values:
R R work R free
0.182 0.18 0.226
Expression systems:
  • Escherichia coli
  • Not provided