PDBe 5om0

X-ray diffraction
3.2Å resolution

CH2 chimera of human 14-3-3 sigma with the Gli1 phosphopeptide around Ser640

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
14-3-3 protein sigma; Zinc finger protein GLI1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 27.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
  • Canonical: P08151 (Residues: 637-645; Coverage: 1%)
  • nullnull
Gene names: GLI, GLI1, HME1, SFN
Sequence domains: 14-3-3 protein

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P6422
Unit cell:
a: 110.426Å b: 110.426Å c: 174.114Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.215 0.267
Expression system: Escherichia coli