PDBe 5okp

X-ray diffraction
1.85Å resolution

Crystal structure of human SHIP2 Phosphatase-C2 double mutant F593D/L597D

Released:

Function and Biology Details

Reaction catalysed:
1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 3,4-diphosphate + phosphate
Biochemical function:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2 Chain: A
Molecule details ›
Chain: A
Length: 461 amino acids
Theoretical weight: 52.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15357 (Residues: 420-878; Coverage: 37%)
Gene names: INPPL1, SHIP2
Sequence domains: Endonuclease/Exonuclease/phosphatase family

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Unit cell:
a: 43.745Å b: 73.426Å c: 157.964Å
α: 90° β: 90.7° γ: 90°
R-values:
R R work R free
0.179 0.178 0.205
Expression system: Escherichia coli BL21(DE3)