PDBe 5ohv

X-ray diffraction
2.8Å resolution

K33-specific affimer bound to K33 diUb

Released:

Function and Biology Details

Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin Chains: A, C
Molecule details ›
Chains: A, C
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
K33-specific affimer Chains: B, D
Molecule details ›
Chains: B, D
Length: 116 amino acids
Theoretical weight: 13.24 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: R3
Unit cell:
a: 120.288Å b: 120.288Å c: 69.973Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.174 0.224
Expression system: Escherichia coli