PDBe 5o6t

X-ray diffraction
1.57Å resolution

BIRC4 RING in complex with dimeric ubiquitin variant

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase XIAP Chains: A, B
Molecule details ›
Chains: A, B
Length: 66 amino acids
Theoretical weight: 7.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P98170 (Residues: 434-497; Coverage: 13%)
Gene names: API3, BIRC4, IAP3, XIAP
Sequence domains: Zinc finger, C3HC4 type (RING finger)
Polyubiquitin-B Chains: C, D
Molecule details ›
Chains: C, D
Length: 80 amino acids
Theoretical weight: 9.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 35.715Å b: 69.972Å c: 109.747Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.156 0.183
Expression system: Escherichia coli BL21(DE3)