PDBe 5nqk

X-ray diffraction
3.25Å resolution

human 199.16 TCR in complex with Melan-A/MART-1 (26-35) peptide and HLA-A2

Released:
Source organism: Homo sapiens
Entry authors: Exertier C, Reiser J-B, Lantez V, Chouquet A, Bonneville M, Saulquin X, Housset D

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A-2 alpha chain Chain: H
Molecule details ›
Chain: H
Length: 276 amino acids
Theoretical weight: 31.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01892 (Residues: 25-300; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Beta-2-microglobulin Chain: L
Molecule details ›
Chain: L
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Melanoma antigen recognized by T-cells 1 Chain: P
Molecule details ›
Chain: P
Length: 10 amino acids
Theoretical weight: 985 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q16655 (Residues: 26-35; Coverage: 9%)
Gene names: MART1, MLANA
T cell receptor alpha constant Chain: A
Molecule details ›
Chain: A
Length: 211 amino acids
Theoretical weight: 23.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01848 (Residues: 1-91; Coverage: 65%)
Gene names: TCRA, TRAC
Sequence domains: Domain of unknown function (DUF1968)
T cell receptor beta variable 19 Chain: B
Molecule details ›
Chain: B
Length: 251 amino acids
Theoretical weight: 28.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A075B6N1 (Residues: 22-113; Coverage: 99%)
Gene name: TRBV19

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 225.18Å b: 49.72Å c: 95.72Å
α: 90° β: 97.69° γ: 90°
R-values:
R R work R free
0.228 0.224 0.316
Expression systems:
  • Escherichia coli
  • Not provided
  • Escherichia coli BL21(DE3)