PDBe 5mtn

X-ray diffraction
2.85Å resolution

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase Lck Chain: A
Molecule details ›
Chain: A
Length: 116 amino acids
Theoretical weight: 13.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P06239 (Residues: 118-231; Coverage: 22%)
Gene name: LCK
Sequence domains: SH2 domain
Monobody Mb(Lck_1) Chain: B
Molecule details ›
Chain: B
Length: 95 amino acids
Theoretical weight: 10.05 KDa
Source organism: synthetic construct
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P3221
Unit cell:
a: 91.397Å b: 91.397Å c: 88.778Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.228 0.265
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'