PDBe 5mj3

X-ray diffraction
1.74Å resolution

INTERLEUKIN-23 COMPLEX WITH AN ANTAGONISTIC ALPHABODY, CRYSTAL FORM 1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Interleukin-12 subunit beta Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 34.74 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P29460 (Residues: 23-328; Coverage: 100%)
Gene names: IL12B, NKSF2
Sequence domains: Cytokine interleukin-12p40 C-terminus
Interleukin-23 subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 179 amino acids
Theoretical weight: 19.81 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9NPF7 (Residues: 20-189; Coverage: 100%)
Gene names: IL23A, SGRF, UNQ2498/PRO5798
Sequence domains: Interleukin 23 subunit alpha
ALPHABODY MA12 Chain: C
Molecule details ›
Chain: C
Length: 118 amino acids
Theoretical weight: 11.95 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments


1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 56.96Å b: 56.74Å c: 100.07Å
α: 90° β: 99.73° γ: 90°
R-values:
R R work R free
0.165 0.165 0.198
Expression systems:
  • Homo sapiens
  • Escherichia coli BL21(DE3)