PDBe 5mcu

X-ray diffraction
1.7Å resolution

New Insights into the Role of DNA Shape on Its Recognition by p53 Proteins (complex p53DBD-LHG2)

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Cellular tumor antigen p53 Chains: A, B
Molecule details ›
Chains: A, B
Length: 200 amino acids
Theoretical weight: 22.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P04637 (Residues: 94-293; Coverage: 51%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
DNA Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.47 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21212
Unit cell:
a: 109.133Å b: 68.422Å c: 69.422Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.201
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided