PDBe 5m35

X-ray diffraction
2.38Å resolution

The molecular tweezer CLR01 stabilizes a disordered protein-protein interface

Released:

Function and Biology Details

Reaction catalysed:
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
14-3-3 protein zeta/delta Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 26.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: NEW P63104 (Residues: 2-230; Coverage: 94%)
Gene name: YWHAZ
Sequence domains: 14-3-3 protein
M-phase inducer phosphatase 3 Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1.27 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: NEW P30307 (Residues: 211-220; Coverage: 2%)
Gene name: CDC25C
M-phase inducer phosphatase 3 Chain: D
Molecule details ›
Chain: D
Length: 7 amino acids
Theoretical weight: 884 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: NEW P30307 (Residues: 213-219; Coverage: 2%)
Gene name: CDC25C

Ligands and Environments

2 bound ligands:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 72.221Å b: 104.075Å c: 114.451Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.224 0.277
Expression systems:
  • Escherichia coli BL21
  • Not provided