PDBe 5m32

Electron Microscopy
3.8Å resolution

Human 26S proteasome in complex with Oprozomib

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 47-mer (preferred)
Entry contents:
34 distinct polypeptide molecules
Macromolecules (34 distinct):
Proteasome subunit alpha type-2 Chains: A, O
Molecule details ›
Chains: A, O
Length: 234 amino acids
Theoretical weight: 25.93 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P25787 (Residues: 1-234; Coverage: 100%)
Gene names: HC3, PSC3, PSMA2
Sequence domains:
Proteasome subunit alpha type-4 Chains: B, P
Molecule details ›
Chains: B, P
Length: 261 amino acids
Theoretical weight: 29.53 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P25789 (Residues: 1-261; Coverage: 100%)
Gene names: HC9, PSC9, PSMA4
Sequence domains:
Proteasome subunit alpha type-7 Chain: C
Molecule details ›
Chain: C
Length: 234 amino acids
Theoretical weight: 26.16 KDa
Source organism: Homo sapiens
UniProt: Gene names: HSPC, PSMA7
Sequence domains:
Proteasome subunit alpha type-5 Chains: D, R
Molecule details ›
Chains: D, R
Length: 241 amino acids
Theoretical weight: 26.44 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P28066 (Residues: 1-241; Coverage: 100%)
Gene name: PSMA5
Sequence domains:
Proteasome subunit alpha type-1 Chain: E
Molecule details ›
Chain: E
Length: 234 amino acids
Theoretical weight: 26.2 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P25786 (Residues: 4-237; Coverage: 89%)
Gene names: HC2, NU, PROS30, PSC2, PSMA1
Sequence domains:
Proteasome subunit alpha type-3 Chains: F, T
Molecule details ›
Chains: F, T
Length: 255 amino acids
Theoretical weight: 28.47 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P25788 (Residues: 1-255; Coverage: 100%)
Gene names: HC8, PSC8, PSMA3
Sequence domains:
Proteasome subunit alpha type-6 Chains: G, U
Molecule details ›
Chains: G, U
Length: 246 amino acids
Theoretical weight: 27.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P60900 (Residues: 1-246; Coverage: 100%)
Gene names: PROS27, PSMA6
Sequence domains:
Proteasome subunit beta type-7 Chains: H, V
Molecule details ›
Chains: H, V
Length: 277 amino acids
Theoretical weight: 30 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW Q99436 (Residues: 1-277; Coverage: 100%)
Gene names: PSMB7, Z
Sequence domains:
Proteasome subunit beta type-3 Chains: I, W
Molecule details ›
Chains: I, W
Length: 205 amino acids
Theoretical weight: 22.97 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P49720 (Residues: 1-205; Coverage: 100%)
Gene name: PSMB3
Sequence domains: Proteasome subunit
Proteasome subunit beta type-2 Chains: J, X
Molecule details ›
Chains: J, X
Length: 196 amino acids
Theoretical weight: 22.35 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P49721 (Residues: 1-196; Coverage: 98%)
Gene name: PSMB2
Sequence domains: Proteasome subunit
Proteasome subunit beta type-5 Chains: K, Y
Molecule details ›
Chains: K, Y
Length: 204 amino acids
Theoretical weight: 22.48 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P28074 (Residues: 60-263; Coverage: 78%)
Gene names: LMPX, MB1, PSMB5, X
Proteasome subunit beta type-1 Chains: L, Z
Molecule details ›
Chains: L, Z
Length: 241 amino acids
Theoretical weight: 26.52 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P20618 (Residues: 1-241; Coverage: 100%)
Gene names: PSC5, PSMB1
Sequence domains: Proteasome subunit
Proteasome subunit beta type-4 Chains: M, a
Molecule details ›
Chains: M, a
Length: 264 amino acids
Theoretical weight: 29.23 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P28070 (Residues: 1-264; Coverage: 100%)
Gene names: PROS26, PSMB4
Sequence domains: Proteasome subunit
Proteasome subunit beta type-6 Chains: N, b
Molecule details ›
Chains: N, b
Length: 239 amino acids
Theoretical weight: 25.38 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P28072 (Residues: 1-239; Coverage: 100%)
Gene names: LMPY, PSMB6, Y
Sequence domains: Proteasome subunit
Proteasome subunit alpha type-7 Chain: Q
Molecule details ›
Chain: Q
Length: 235 amino acids
Theoretical weight: 26.35 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW O14818 (Residues: 2-236; Coverage: 95%)
Gene names: HSPC, PSMA7
Sequence domains:
Proteasome subunit alpha type-1 Chain: S
Molecule details ›
Chain: S
Length: 238 amino acids
Theoretical weight: 26.63 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P25786 (Residues: 2-239; Coverage: 91%)
Gene names: HC2, NU, PROS30, PSC2, PSMA1
Sequence domains:
26S proteasome regulatory subunit 7 Chain: c
Molecule details ›
Chain: c
Length: 433 amino acids
Theoretical weight: 48.7 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P35998 (Residues: 1-433; Coverage: 100%)
Gene names: MSS1, PSMC2
Sequence domains:
26S proteasome regulatory subunit 4 Chain: d
Molecule details ›
Chain: d
Length: 428 amino acids
Theoretical weight: 47.91 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P62191 (Residues: 1-428; Coverage: 97%)
Gene name: PSMC1
Sequence domains:
26S proteasome regulatory subunit 6B Chain: e
Molecule details ›
Chain: e
Length: 418 amino acids
Theoretical weight: 47.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P43686 (Residues: 1-418; Coverage: 100%)
Gene names: MIP224, PSMC4, TBP7
Sequence domains:
26S proteasome regulatory subunit 10B Chain: f
Molecule details ›
Chain: f
Length: 379 amino acids
Theoretical weight: 43.07 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P62333 (Residues: 1-379; Coverage: 97%)
Gene names: PSMC6, SUG2
Sequence domains:
26S proteasome regulatory subunit 6A Chain: g
Molecule details ›
Chain: g
Length: 439 amino acids
Theoretical weight: 49.27 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P17980 (Residues: 1-439; Coverage: 100%)
Gene names: PSMC3, TBP1
Sequence domains:
26S proteasome regulatory subunit 8 Chain: h
Molecule details ›
Chain: h
Length: 355 amino acids
Theoretical weight: 39.82 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P62195 (Residues: 38-392; Coverage: 87%)
Gene names: PSMC5, SUG1
Sequence domains:
26S proteasome non-ATPase regulatory subunit 1 Chain: i
Molecule details ›
Chain: i
Length: 953 amino acids
Theoretical weight: 105.96 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW Q99460 (Residues: 1-953; Coverage: 100%)
Gene name: PSMD1
Sequence domains:
26S proteasome non-ATPase regulatory subunit 3 Chain: j
Molecule details ›
Chain: j
Length: 534 amino acids
Theoretical weight: 61.07 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW O43242 (Residues: 1-534; Coverage: 100%)
Gene name: PSMD3
Sequence domains:
26S proteasome non-ATPase regulatory subunit 12 Chain: k
Molecule details ›
Chain: k
Length: 456 amino acids
Theoretical weight: 52.98 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW O00232 (Residues: 1-456; Coverage: 100%)
Gene name: PSMD12
Sequence domains:
26S proteasome non-ATPase regulatory subunit 11 Chain: l
Molecule details ›
Chain: l
Length: 422 amino acids
Theoretical weight: 47.53 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW O00231 (Residues: 1-422; Coverage: 100%)
Gene name: PSMD11
Sequence domains:
26S proteasome non-ATPase regulatory subunit 6 Chain: m
Molecule details ›
Chain: m
Length: 389 amino acids
Theoretical weight: 45.59 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW Q15008 (Residues: 1-389; Coverage: 100%)
Gene names: KIAA0107, PFAAP4, PSMD6
Sequence domains:
26S proteasome non-ATPase regulatory subunit 7 Chain: n
Molecule details ›
Chain: n
Length: 324 amino acids
Theoretical weight: 37.09 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P51665 (Residues: 1-324; Coverage: 100%)
Gene names: MOV34L, PSMD7
Sequence domains:
26S proteasome non-ATPase regulatory subunit 13 Chain: o
Molecule details ›
Chain: o
Length: 376 amino acids
Theoretical weight: 43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW Q9UNM6 (Residues: 1-376; Coverage: 100%)
Gene name: PSMD13
Sequence domains: PCI domain
26S proteasome non-ATPase regulatory subunit 4 Chain: p
Molecule details ›
Chain: p
Length: 377 amino acids
Theoretical weight: 40.78 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P55036 (Residues: 1-377; Coverage: 100%)
Gene names: MCB1, PSMD4
Sequence domains:
26S proteasome non-ATPase regulatory subunit 14 Chain: q
Molecule details ›
Chain: q
Length: 310 amino acids
Theoretical weight: 34.62 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW O00487 (Residues: 1-310; Coverage: 100%)
Gene names: POH1, PSMD14
Sequence domains:
26S proteasome non-ATPase regulatory subunit 8 Chain: r
Molecule details ›
Chain: r
Length: 350 amino acids
Theoretical weight: 39.67 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P48556 (Residues: 1-350; Coverage: 100%)
Gene name: PSMD8
Sequence domains: CSN8/PSMD8/EIF3K family
26S proteasome complex subunit SEM1 Chain: s
Molecule details ›
Chain: s
Length: 70 amino acids
Theoretical weight: 8.28 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: NEW P60896 (Residues: 1-70; Coverage: 100%)
Gene names: C7orf76, DSS1, SEM1, SHFDG1, SHFM1
Sequence domains: DSS1/SEM1 family
bound Oprozomib Chains: t, u
Molecule details ›
Chains: t, u
Length: 4 amino acids
Theoretical weight: 537 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-4146
Expression system: Not provided