PDBe 5m2i

X-ray diffraction
2.15Å resolution

Structure of human Tumor Necrosis Factor (TNF) in complex with the Llama VHH1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tumor necrosis factor, soluble form Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 157 amino acids
Theoretical weight: 17.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01375 (Residues: 77-233; Coverage: 67%)
Gene names: TNF, TNFA, TNFSF2
Sequence domains: TNF(Tumour Necrosis Factor) family
VHH1 Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 121 amino acids
Theoretical weight: 13.42 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 110.33Å b: 117.41Å c: 141.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.207 0.238
Expression system: Escherichia coli BL21