PDBe 5lsk

X-ray diffraction
3.5Å resolution

CRYSTAL STRUCTURE OF THE HUMAN KINETOCHORE MIS12-CENP-C COMPLEX

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Centromere protein C Chain: P
Molecule details ›
Chain: P
Length: 76 amino acids
Theoretical weight: 8.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q03188 (Residues: 1-71; Coverage: 8%)
Gene names: CENPC, CENPC1, ICEN7
Protein MIS12 homolog Chain: A
Molecule details ›
Chain: A
Length: 205 amino acids
Theoretical weight: 24.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H081 (Residues: 1-205; Coverage: 100%)
Gene name: MIS12
Sequence domains: Mis12 protein
Polyamine-modulated factor 1 Chain: B
Molecule details ›
Chain: B
Length: 176 amino acids
Theoretical weight: 20.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6P1K2 (Residues: 31-205; Coverage: 85%)
Gene name: PMF1
Sequence domains: Nnf1
Kinetochore-associated protein DSN1 homolog Chain: D
Molecule details ›
Chain: D
Length: 296 amino acids
Theoretical weight: 33.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H410 (Residues: 67-356; Coverage: 82%)
Gene names: C20orf172, DSN1, MIS13
Sequence domains: Mis12-Mtw1 protein family
Kinetochore-associated protein NSL1 homolog Chain: N
Molecule details ›
Chain: N
Length: 206 amino acids
Theoretical weight: 23.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96IY1 (Residues: 1-206; Coverage: 73%)
Gene names: C1orf48, DC31, DC8, MIS14, NSL1
Sequence domains: Kinetochore protein Mis14 like

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 146.01Å b: 112.69Å c: 90.81Å
α: 90° β: 114.18° γ: 90°
R-values:
R R work R free
0.247 0.244 0.298
Expression system: Escherichia coli BL21(DE3)