PDBe 5lqf

X-ray diffraction
2.06Å resolution

Function and Biology Details

Reactions catalysed:
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cyclin-dependent kinase 1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 302 amino acids
Theoretical weight: 34.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06493 (Residues: 1-297; Coverage: 100%)
Gene names: CDC2, CDC28A, CDK1, CDKN1, P34CDC2
Sequence domains: Protein kinase domain
Structure domains:
G2/mitotic-specific cyclin-B1 Chains: B, E
Molecule details ›
Chains: B, E
Length: 273 amino acids
Theoretical weight: 31.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14635 (Residues: 165-433; Coverage: 62%)
Gene names: CCNB, CCNB1
Sequence domains:
Structure domains: Cyclin-like
Cyclin-dependent kinases regulatory subunit 2 Chains: C, F
Molecule details ›
Chains: C, F
Length: 84 amino acids
Theoretical weight: 10.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P33552 (Residues: 1-79; Coverage: 100%)
Gene name: CKS2
Sequence domains: Cyclin-dependent kinase regulatory subunit
Structure domains: Cyclin-dependent kinase, regulatory subunit

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P1
Unit cell:
a: 65.04Å b: 67.75Å c: 85.06Å
α: 103.88° β: 90.89° γ: 90.42°
R-values:
R R work R free
0.2 0.198 0.254
Expression system: Escherichia coli