› 5lg7

Solution NMR

Solution NMR structure of Tryptophan to Arginine mutant of Arkadia RING domain

Released:
DOI: 10.2210/pdb5lg7/pdb
PDB entry 5lg7 coloured by chain, ensemble of 31 models, front view.
PDB entry 5lg7 coloured by chain, ensemble of 31 models, side view.
PDB entry 5lg7 coloured by chain, ensemble of 31 models, top view.
Source organism: Homo sapiens
Primary publication:
A Residue Specific Insight into the Arkadia E3 Ubiquitin Ligase Activity and Conformational Plasticity.
Birkou M, Chasapis CT, Marousis KD, Loutsidou AK, Bentrop D, Lelli M, Herrmann T, Carthy JM, Episkopou V, Spyroulias GA
J Mol Biol 429 2373-2386 (2017)
PMID: 28647409

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180588 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase Arkadia Chain: A
Molecule details ›
Chain: A
Length: 69 amino acids
Theoretical weight: 7.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6ZNA4 (Residues: 927-994; Coverage: 7%)
Gene name: RNF111
Sequence domains: Ring finger domain

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 49%
Refinement method: molecular mechanics
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Recent aspects of the effects of zinc on human health.
Chasapis et al. (2020)
5 more

1 mention without citation

SUMO-Targeted Ubiquitin Ligases and Their Functions in Maintaining Genome Stability.
Chang et al. (2021)