PDBe 5lg7

Solution NMR

Solution NMR structure of Tryptophan to Arginine mutant of Arkadia RING domain

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase Arkadia Chain: A
Molecule details ›
Chain: A
Length: 69 amino acids
Theoretical weight: 7.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6ZNA4 (Residues: 927-994; Coverage: 7%)
Gene name: RNF111
Sequence domains: Ring finger domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 53%
Refinement method: molecular mechanics
Chemical shifts: BMR25248  
Expression system: Escherichia coli BL21(DE3)