PDBe 5lax

X-ray diffraction
2.6Å resolution

Crystal structure of HLA_DRB1*04:01 in complex with alpha-enolase peptide 26-40

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class II histocompatibility antigen, DR alpha chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 189 amino acids
Theoretical weight: 21.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P01903 (Residues: 26-206; Coverage: 79%)
Gene names: HLA-DRA, HLA-DRA1
Sequence domains:
Structure domains:
HLA class II histocompatibility antigen, DRB1-4 beta chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 198 amino acids
Theoretical weight: 23.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P13760 (Residues: 30-219; Coverage: 80%)
Gene name: HLA-DRB1
Sequence domains:
Structure domains:
Alpha-enolase Chains: E, F
Molecule details ›
Chains: E, F
Length: 15 amino acids
Theoretical weight: 1.45 KDa
Source organism: Homo sapiens
Expression system: synthetic construct
UniProt:
  • Canonical: NEW P06733 (Residues: 26-40; Coverage: 4%)
Gene names: ENO1, ENO1L1, MBPB1, MPB1

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 69.176Å b: 73.45Å c: 144.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.191 0.27
Expression systems:
  • Escherichia coli BL21(DE3)
  • synthetic construct