PDBe 5l8w

X-ray diffraction
2.79Å resolution

Structure of USP12-UB-PRG/UAF1

Released:

Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ubiquitin carboxyl-terminal hydrolase 12 Chain: A
Molecule details ›
Chain: A
Length: 376 amino acids
Theoretical weight: 43.74 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O75317 (Residues: 1-370; Coverage: 100%)
Gene names: UBH1, USP12, USP12L1
Sequence domains: Ubiquitin carboxyl-terminal hydrolase
WD repeat-containing protein 48 Chain: B
Molecule details ›
Chain: B
Length: 580 amino acids
Theoretical weight: 65.19 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8TAF3 (Residues: 9-580; Coverage: 85%)
  • Best match: Q8TAF3-2 (Residues: 1-22)
Gene names: KIAA1449, UAF1, WDR48
Sequence domains: WD domain, G-beta repeat
Ubiquitin Chain: C
Molecule details ›
Chain: C
Length: 75 amino acids
Theoretical weight: 8.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG47 (Residues: 153-227; Coverage: 33%)
Gene name: UBB
Sequence domains: Ubiquitin family

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: I222
Unit cell:
a: 103.679Å b: 152.82Å c: 182.932Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.208 0.259
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli