PDBe 5l0m

X-ray diffraction
2.2Å resolution

hLRH-1 DNA Binding Domain - 12bp Oct4 promoter complex

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Nuclear receptor subfamily 5 group A member 2 Chain: A
Molecule details ›
Chain: A
Length: 112 amino acids
Theoretical weight: 13.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00482 (Residues: 79-187; Coverage: 20%)
Gene names: B1F, CPF, FTF, NR5A2
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
DNA (5'-D(*CP*TP*AP*GP*CP*CP*TP*TP*GP*AP*CP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.6 KDa
Source organism: Mus musculus
Expression system: Not provided
DNA (5'-D(*GP*GP*TP*CP*AP*AP*GP*GP*CP*TP*AP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.73 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P43
Unit cell:
a: 40.931Å b: 40.931Å c: 105.055Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.154 0.197
Expression systems:
  • Escherichia coli
  • Not provided