PDBe 5ksa

X-ray diffraction
2Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class II histocompatibility antigen, DQ alpha 1 chain Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 21.5 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P01909 (Residues: 24-206; Coverage: 79%)
Gene name: HLA-DQA1
Sequence domains:
Structure domains:
Gamma-gliadin; HLA class II histocompatibility antigen, DQ beta 1 chain Chain: B
Molecule details ›
Chain: B
Length: 225 amino acids
Theoretical weight: 25.83 KDa
Source organisms: Expression system: Trichoplusia ni
UniProt:
  • Canonical: P08079 (Residues: 132-141; Coverage: 4%)
  • Canonical: A0A0U5Q247 (Residues: 12-12, 33-224; Coverage: 75%)
  • nullnull
Gene name: HLA-DQB1
Sequence domains:
Structure domains:
T cell receptor alpha constant; T cell receptor alpha variable 20 Chain: C
Molecule details ›
Chain: C
Length: 206 amino acids
Theoretical weight: 23.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0B4J274 (Residues: 22-112; Coverage: 100%)
  • Canonical: P01848 (Residues: 1-93; Coverage: 66%)
  • nullnull
Gene names: TCRA, TRAC, TRAV20
Sequence domains:
Structure domains: Immunoglobulins
T cell receptor beta constant 2; V_segment translation product Chain: D
Molecule details ›
Chain: D
Length: 243 amino acids
Theoretical weight: 27.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A5B9 (Residues: 1-129; Coverage: 73%)
  • Canonical: A0A580 (Residues: 22-114; Coverage: 100%)
  • nullnull
Gene names: TCRBC2, TCRBV1S1A1N1, TRBC2, TRBV9
Sequence domains:
Structure domains: Immunoglobulins
Gamma-gliadin Chain: J
Molecule details ›
Chain: J
Length: 11 amino acids
Theoretical weight: 1.29 KDa
Source organism: Triticum aestivum
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P08079 (Residues: 132-141; Coverage: 4%)

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 62.548Å b: 98.831Å c: 80.175Å
α: 90° β: 95.69° γ: 90°
R-values:
R R work R free
0.171 0.169 0.206
Expression systems:
  • Trichoplusia ni
  • Escherichia coli