PDBe 5kji

X-ray diffraction
2.71Å resolution

Crystal structure of an active polycomb repressive complex 2 in the basal state

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
putative polycomb protein Eed Chain: A
Molecule details ›
Chain: A
Length: 605 amino acids
Theoretical weight: 66.02 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: G0S8H7 (Residues: 1-565; Coverage: 100%)
Gene name: CTHT_0029920
Sequence domains: WD domain, G-beta repeat
Zinc finger domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 937 amino acids
Theoretical weight: 106.87 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: G0RYC6 (Residues: 530-691; Coverage: 22%)
  • Canonical: G0SDW4 (Residues: 191-950; Coverage: 50%)
  • nullnull
Gene names: CTHT_0006210, CTHT_0053230
Sequence domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 82.333Å b: 74.619Å c: 127.035Å
α: 90° β: 105.63° γ: 90°
R-values:
R R work R free
0.183 0.179 0.249
Expression system: Saccharomyces cerevisiae S288C