PDBe 5jzi

X-ray diffraction
2.5Å resolution

Crystal structure of 1406 TCR bound to HLA-A2 with HCV 1406-1415 antigen peptide

Released:

Function and Biology Details

Reactions catalysed:
ATP + H(2)O = ADP + phosphate
NTP + H(2)O = NDP + phosphate
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 20-mer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A-2 alpha chain Chains: A, F
Molecule details ›
Chains: A, F
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01892 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Beta-2-microglobulin Chains: B, G
Molecule details ›
Chains: B, G
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Serine protease NS3 Chains: C, H
Molecule details ›
Chains: C, H
Length: 10 amino acids
Theoretical weight: 998 Da
Source organism: Hepacivirus C
Expression system: Not provided
UniProt:
  • Canonical: P27958 (Residues: 1406-1415; Coverage: 0%)
HCV1406 TCR alpha chain Chains: D, I
Molecule details ›
Chains: D, I
Length: 211 amino acids
Theoretical weight: 23.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
HCV1406 TCR beta chain Chains: E, J
Molecule details ›
Chains: E, J
Length: 245 amino acids
Theoretical weight: 28.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P3121
Unit cell:
a: 128.76Å b: 128.76Å c: 223.59Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.196 0.232
Expression systems:
  • Escherichia coli
  • Not provided
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'