PDBe 5jz7

X-ray diffraction
3.4Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Beta-nerve growth factor Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 110 amino acids
Theoretical weight: 12.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01138 (Residues: 128-237; Coverage: 49%)
Gene names: NGF, NGFB
Structure domains: Cystine-knot cytokines
MEDI578 scFv, heavy chain Chains: C, G, H, J
Molecule details ›
Chains: C, G, H, J
Length: 130 amino acids
Theoretical weight: 13.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
MEDI578 scFv, light chain Chains: D, I, K, L
Molecule details ›
Chains: D, I, K, L
Length: 122 amino acids
Theoretical weight: 12.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: I4
Unit cell:
a: 182.085Å b: 182.085Å c: 109.832Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.258 0.258 0.268
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli