PDBe 5jpq

Electron Microscopy
7.3Å resolution

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + pseudouridine(1191) in yeast 18S rRNA = S-adenosyl-L-homocysteine + N(1)-methylpseudouridine(1191) in yeast 18S rRNA. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 56-mer (preferred)
Entry contents:
34 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (36 distinct):
40S ribosomal protein S3a Chain: o
Molecule details ›
Chain: o
Length: 265 amino acids
Theoretical weight: 29.71 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: Q23DE3 (Residues: 1-265; Coverage: 100%)
Gene name: TTHERM_00047480
Sequence domains: Ribosomal S3Ae family
40S ribosomal protein S4 Chain: p
Molecule details ›
Chain: p
Length: 259 amino acids
Theoretical weight: 29.51 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: P0C233 (Residues: 1-259; Coverage: 100%)
Sequence domains:
40S ribosomal protein S6 Chain: r
Molecule details ›
Chain: r
Length: 293 amino acids
Theoretical weight: 32.61 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: A4VD76 (Residues: 1-293; Coverage: 100%)
Gene name: TTHERM_00149798
Sequence domains: Ribosomal protein S6e
40S ribosomal protein S7 Chain: s
Molecule details ›
Chain: s
Length: 197 amino acids
Theoretical weight: 23.23 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: I7MD19 (Residues: 1-197; Coverage: 100%)
Gene name: TTHERM_00471090
Sequence domains: Ribosomal protein S7e
40S ribosomal protein S8 Chain: t
Molecule details ›
Chain: t
Length: 208 amino acids
Theoretical weight: 24.25 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: Q22AV0 (Residues: 1-208; Coverage: 100%)
Gene name: TTHERM_01151600
Sequence domains: Ribosomal protein S8e
40S ribosomal protein S9-A Chain: u
Molecule details ›
Chain: u
Length: 197 amino acids
Theoretical weight: 22.49 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: O13516 (Residues: 1-197; Coverage: 100%)
Gene names: RPS13A, RPS9A, YPL081W, YS11A
Sequence domains: S4 domain
40S ribosomal protein S5 Chain: q
Molecule details ›
Chain: q
Length: 225 amino acids
Theoretical weight: 25.07 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: P26783 (Residues: 1-225; Coverage: 100%)
Gene names: J2045, RPS2, RPS5, YJR123W
Sequence domains: Ribosomal protein S7p/S5e
40S ribosomal protein S22-A Chain: z
Molecule details ›
Chain: z
Length: 130 amino acids
Theoretical weight: 14.65 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: P0C0W1 (Residues: 1-130; Coverage: 100%)
Gene names: J0355, RPS22A, RPS24, RPS24A, YJL190C
Sequence domains: Ribosomal protein S8
40S ribosomal protein S16-A Chain: x
Molecule details ›
Chain: x
Length: 143 amino acids
Theoretical weight: 15.88 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: P0CX51 (Residues: 1-143; Coverage: 100%)
Gene names: RP61R, RPS16A, YM9375.12, YMR143W
Sequence domains: Ribosomal protein S9/S16
Ribosome biogenesis protein BMS1 Chain: a
Molecule details ›
Chain: a
Length: 1183 amino acids
Theoretical weight: 135.79 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: Q08965 (Residues: 1-1183; Coverage: 100%)
Gene names: BMS1, YPL217C
Sequence domains:
Ribosomal RNA small subunit methyltransferase NEP1 Chains: e, f
Molecule details ›
Chains: e, f
Length: 252 amino acids
Theoretical weight: 27.94 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: Q06287 (Residues: 1-252; Coverage: 100%)
Gene names: EMG1, L9470.5, NEP1, YLR186W
Sequence domains: EMG1/NEP1 methyltransferase
Imp3 Chain: b
Molecule details ›
Chain: b
Length: 183 amino acids
Theoretical weight: 21.8 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: G0SDL4 (Residues: 1-183; Coverage: 100%)
Gene name: CTHT_0052200
Sequence domains:
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase Chains: W, X
Molecule details ›
Chains: W, X
Length: 232 amino acids
Theoretical weight: 26.46 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: P58032 (Residues: 1-232; Coverage: 100%)
Gene names: C33_014, SSO0940, flpA
Sequence domains: Fibrillarin
RNA 3'-terminal phosphate cyclase-like protein Chain: Z
Molecule details ›
Chain: Z
Length: 367 amino acids
Theoretical weight: 40.22 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: Q08096 (Residues: 1-367; Coverage: 100%)
Gene names: RCL1, RTC1, YOL010W
Sequence domains:
UTP6 Chain: Q
Molecule details ›
Chain: Q
Length: 456 amino acids
Theoretical weight: 38.83 KDa
Source organism: Chaetomium thermophilum
40S ribosomal protein S24 Chain: 0
Molecule details ›
Chain: 0
Length: 149 amino acids
Theoretical weight: 17.13 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: I7MAL3 (Residues: 1-149; Coverage: 100%)
Gene name: TTHERM_00467660
Sequence domains: Ribosomal protein S24e
40S ribosomal protein S28-A Chain: 1
Molecule details ›
Chain: 1
Length: 67 amino acids
Theoretical weight: 7.61 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: Q3E7X9 (Residues: 1-67; Coverage: 100%)
Gene names: O3600, RPS28A, RPS33, RPS33A, YOR167C
Sequence domains: Ribosomal protein S28e
Ribosomal RNA-processing protein 9 Chain: Y
Molecule details ›
Chain: Y
Length: 573 amino acids
Theoretical weight: 65.15 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: Q06506 (Residues: 1-573; Coverage: 100%)
Gene names: RRP9, YPR137W
Sequence domains: WD domain, G-beta repeat
40S ribosomal protein S14-A Chain: w
Molecule details ›
Chain: w
Length: 137 amino acids
Theoretical weight: 14.56 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: P06367 (Residues: 1-137; Coverage: 100%)
Gene names: CRY1, RPL59, RPS14A, YCR031C, YCR31C
Sequence domains: Ribosomal protein S11
40S ribosomal protein S13 Chain: v
Molecule details ›
Chain: v
Length: 151 amino acids
Theoretical weight: 17.06 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: P05756 (Residues: 1-151; Coverage: 100%)
Gene names: D4252, RPS13, RPS13C, YD9609.18, YDR064W
Sequence domains:
40S ribosomal protein S11, putative Chain: y
Molecule details ›
Chain: y
Length: 157 amino acids
Theoretical weight: 18.14 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: Q22B78 (Residues: 1-157; Coverage: 100%)
Gene name: TTHERM_01109770
Sequence domains:
RNA cytidine acetyltransferase Chains: i, j
Molecule details ›
Chains: i, j
Length: 1073 amino acids
Theoretical weight: 119.8 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: G0S273 (Residues: 1-1073; Coverage: 100%)
Gene names: CTHT_0016220, NAT10
Sequence domains:
50S ribosomal protein L7Ae Chains: U, V
Molecule details ›
Chains: U, V
Length: 130 amino acids
Theoretical weight: 14.07 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: P55858 (Residues: 1-127; Coverage: 100%)
Gene names: C04_031, SSO0091, rpl7ae
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
UTP10 Chain: G
Molecule details ›
Chain: G
Length: 1802 amino acids
Theoretical weight: 198.51 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: G0S5L1 (Residues: 1-1802; Coverage: 100%)
Gene name: CTHT_0024640
Sequence domains:
Utp24 Chain: d
Molecule details ›
Chain: d
Length: 184 amino acids
Theoretical weight: 21.1 KDa
Source organism: Tragopogon ruthenicus
UniProt:
  • Canonical: G0SE30 (Residues: 1-184; Coverage: 100%)
Gene name: CTHT_0062220
Sequence domains: Fcf1
Utp30 Chain: k
Molecule details ›
Chain: k
Length: 391 amino acids
Theoretical weight: 43.7 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: G0S7X0 (Residues: 1-391; Coverage: 100%)
Gene name: CTHT_0037280
Sequence domains: Ribosomal protein L1p/L10e family
WD40 domain proteins Chains: A, B, C, D, E, F, J, K, L, N, P, l, n
Molecule details ›
Chains: A, B, C, D, E, F, J, K, L, N, P, l, n
Length: 1290 amino acids
Theoretical weight: 109.8 KDa
Source organism: Chaetomium thermophilum
WD40 domain proteins Chains: M, O, m
Molecule details ›
Chains: M, O, m
Length: 870 amino acids
Theoretical weight: 74.06 KDa
Source organism: Chaetomium thermophilum
C/D box methylation guide ribonucleoprotein complex aNOP56 subunit Chains: S, T
Molecule details ›
Chains: S, T
Length: 412 amino acids
Theoretical weight: 46.77 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: A0A0E3MJI1 (Residues: 1-411; Coverage: 100%)
Gene names: SULA_1947, SULB_1948, SULC_1946
Sequence domains: snoRNA binding domain, fibrillarin
UTP-A oligomerization domain Chain: H
Molecule details ›
Chain: H
Length: 920 amino acids
Theoretical weight: 78.31 KDa
Source organism: Chaetomium thermophilum
UTP-B oligomerisation domain Chain: R
Molecule details ›
Chain: R
Length: 560 amino acids
Theoretical weight: 47.68 KDa
Source organism: Chaetomium thermophilum
KRR1 small subunit processome component Chains: g, h
Molecule details ›
Chains: g, h
Length: 322 amino acids
Theoretical weight: 37.5 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
UniProt:
  • Canonical: G0S3V7 (Residues: 1-322; Coverage: 100%)
Gene name: CTHT_0039040
U3 small nucleolar RNA-associated protein 21 Chain: I
Molecule details ›
Chain: I
Length: 939 amino acids
Theoretical weight: 104.93 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q06078 (Residues: 1-939; Coverage: 100%)
Gene names: L8084.22, UTP21, YLR409C
Sequence domains:
Putative U3 small nucleolar ribonucleoprotein Chain: c
Molecule details ›
Chain: c
Length: 297 amino acids
Theoretical weight: 34.22 KDa
Source organism: Chaetomium thermophilum
UniProt:
  • Canonical: G0SE90 (Residues: 1-297; Coverage: 100%)
Gene name: CTHT_0062900
Sequence domains: Brix domain
U3 RNA Chain: 3
Molecule details ›
Chain: 3
Length: 274 nucleotides
Theoretical weight: 87.97 KDa
18S Ribosomal RNA Chain: 2
Molecule details ›
Chain: 2
Length: 1800 nucleotides
Theoretical weight: 579.76 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.3Å
Relevant EMDB volumes: EMD-8143