PDBe 5jja

X-ray diffraction
2.35Å resolution

Crystal structure of a PP2A B56gamma/BubR1 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform Chains: A, B
Molecule details ›
Chains: A, B
Length: 352 amino acids
Theoretical weight: 41.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13362 (Residues: 30-380; Coverage: 67%)
Gene names: KIAA0044, PPP2R5C
Structure domains: Leucine-rich Repeat Variant
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta Chains: C, D
Molecule details ›
Chains: C, D
Length: 75 amino acids
Theoretical weight: 8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60566 (Residues: 647-720; Coverage: 7%)
Gene names: BUB1B, BUBR1, MAD3L, SSK1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 87.017Å b: 95.588Å c: 167.372Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.204
Expression system: Escherichia coli