› 5jbd

X-ray diffraction
1.8Å resolution

4,6-alpha-glucanotransferase GTFB from Lactobacillus reuteri 121

Released:
DOI: 10.2210/pdb5jbd/pdb
PDB entry 5jbd coloured by chain, front view.
PDB entry 5jbd coloured by chain, side view.
PDB entry 5jbd coloured by chain, top view.
Source organism: Limosilactobacillus reuteri
Primary publication:
Crystal Structure of 4,6-α-Glucanotransferase Supports Diet-Driven Evolution of GH70 Enzymes from α-Amylases in Oral Bacteria.
Bai Y, Gangoiti J, Dijkstra BW, Dijkhuizen L, Pijning T
Structure 25 231-242 (2017)
PMID: 28065507

Function and Biology Details

Reaction catalysed: 
Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177813 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
dextransucrase Chains: A, B
Molecule details ›
Chains: A, B
Length: 893 amino acids
Theoretical weight: 99.51 KDa
Source organism: Limosilactobacillus reuteri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SBM0 (Residues: 735-1619; Coverage: 56%)
Sequence domains: Glycosyl hydrolase family 70
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand CA 2 x CA
Ligand ACT 5 x ACT
Ligand SO4 4 x SO4
Ligand GOL 9 x GOL
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 219.244Å b: 57.852Å c: 150.701Å
α: 90° β: 114.75° γ: 90°
R-values:
R R work R free
0.179 0.177 0.208
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Oral Biofilms: Pathogens, Matrix, and Polymicrobial Interactions in Microenvironments.
Bowen et al. (2018)
3 more

2 mentions without citation

Mining novel starch-converting Glycoside Hydrolase 70 enzymes from the Nestlé Culture Collection genome database: The Lactobacillus reuteri NCC 2613 GtfB.
Gangoiti et al. (2017)
1 more