PDBe 5ja4

X-ray diffraction
2.42Å resolution

Crystal structure of human TONSL and MCM2 HBDs binding to a histone H3-H4 tetramer

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Histone H3.3 Chain: A
Molecule details ›
Chain: A
Length: 79 amino acids
Theoretical weight: 9.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P84243 (Residues: 58-136; Coverage: 58%)
Gene names: H3.3A, H3.3B, H3F3, H3F3A, H3F3B, PP781
Structure domains: Histone, subunit A
Histone H4 Chain: B
Molecule details ›
Chain: B
Length: 102 amino acids
Theoretical weight: 11.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62805 (Residues: 2-103; Coverage: 99%)
Gene names: H4/A, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, HIST4H4
Sequence domains: Centromere kinetochore component CENP-T histone fold
Structure domains: Histone, subunit A
DNA replication licensing factor MCM2 Chain: C
Molecule details ›
Chain: C
Length: 70 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49736 (Residues: 61-130; Coverage: 8%)
Gene names: BM28, CCNL1, CDCL1, KIAA0030, MCM2
Tonsoku-like protein Chain: D
Molecule details ›
Chain: D
Length: 181 amino acids
Theoretical weight: 20.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96HA7 (Residues: 512-692; Coverage: 13%)
Gene names: IKBR, NFKBIL2, TONSL
Sequence domains: Ankyrin repeat
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P321
Unit cell:
a: 139.506Å b: 139.506Å c: 72.896Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.201 0.246
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli