PDBe 5j7g

X-ray diffraction
1.85Å resolution

Structure of MDM2 with low molecular weight inhibitor with aliphatic linker.

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase Mdm2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 109 amino acids
Theoretical weight: 12.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00987 (Residues: 18-125; Coverage: 22%)
Gene name: MDM2
Sequence domains: SWIB/MDM2 domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Unit cell:
a: 36.75Å b: 74.301Å c: 171.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.234
Expression system: Escherichia coli