PDBe 5j3v

X-ray diffraction
3.05Å resolution

Crystal structure of human Karyopherin-beta2 bound to the histone H3 tail

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transportin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 854 amino acids
Theoretical weight: 96.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW Q92973 (Residues: 9-331, 375-898; Coverage: 94%)
Gene names: KPNB2, MIP1, TNPO1, TRN
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Histone H3.1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 17 amino acids
Theoretical weight: 1.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P68431 (Residues: 12-28; Coverage: 13%)
Gene names: H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 150.296Å b: 154.185Å c: 192.661Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.256
Expression system: Escherichia coli