PDBe 5j3h

X-ray diffraction
3.27Å resolution

Human insulin receptor domains L1-CR in complex with peptide S519C16 and 83-7 Fv

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Peptide S519C16 Chain: B
Molecule details ›
Chain: B
Length: 16 amino acids
Theoretical weight: 1.95 KDa
Source organism: Endothia gyrosa
Expression system: Not provided
mAb 83-7 Fab heavy chain Chain: C
Molecule details ›
Chain: C
Length: 123 amino acids
Theoretical weight: 13.44 KDa
Source organism: Mus musculus
Expression system: Brevibacillus
Structure domains: Immunoglobulins
mAb 83-7 Fab light chain Chain: D
Molecule details ›
Chain: D
Length: 118 amino acids
Theoretical weight: 13.15 KDa
Source organism: Mus musculus
Expression system: Brevibacillus
Structure domains: Immunoglobulins
Insulin receptor subunit alpha Chain: E
Molecule details ›
Chain: E
Length: 317 amino acids
Theoretical weight: 36.23 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P06213 (Residues: 28-337; Coverage: 23%)
Gene name: INSR
Sequence domains: Receptor L domain
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P41212
Unit cell:
a: 176.776Å b: 176.776Å c: 86.186Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.226 0.248
Expression systems:
  • Not provided
  • Brevibacillus
  • Cricetulus griseus