› 5j0h

X-ray diffraction
1.64Å resolution

De novo design of protein homo-oligomers with modular hydrogen bond network-mediated specificity

Released:
DOI: 10.2210/pdb5j0h/pdb
PDB entry 5j0h coloured by chain, front view.
PDB entry 5j0h coloured by chain, side view.
PDB entry 5j0h coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
De novo design of protein homo-oligomers with modular hydrogen-bond network-mediated specificity.
Boyken SE, Chen Z, Groves B, Langan RA, Oberdorfer G, Ford A, Gilmore JM, Xu C, DiMaio F, Pereira JH, Sankaran B, Seelig G, Zwart PH, Baker D
Science 352 680-7 (2016)
PMID: 27151862

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165834 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Design construct 2L6HC3_13 Chains: A, B
Molecule details ›
Chains: A, B
Length: 79 amino acids
Theoretical weight: 9.14 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: R32
Unit cell:
a: 53.194Å b: 53.194Å c: 278.642Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.178 0.207
Expression system: Escherichia coli

Quick links

Citations

34 review citations

The coming of age of de novo protein design.
Huang et al. (2016)
33 more

5 mentions without citation

De novo design of protein homo-oligomers with modular hydrogen-bond network-mediated specificity.
Boyken et al. (2016)
4 more