PDBe 5iek

X-ray diffraction
1.8Å resolution

Structure of HLA-B*40:02 in complex with the endogenous peptide REFSKEPEL

Released:
Source organism: Homo sapiens
Entry authors: Alpizar A, Marcilla M, Santiago C

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, B-40 alpha chain Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 32.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q04826 (Residues: 25-300; Coverage: 82%)
Gene names: HLA-B, HLAB
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Inner centromere protein Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.14 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9NQS7 (Residues: 47-55; Coverage: 1%)
Gene name: INCENP

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P212121
Unit cell:
a: 51.26Å b: 82.201Å c: 111.145Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.162 0.188
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided