› 5idj

X-ray diffraction
3.02Å resolution

Bifunctional histidine kinase CckA (domains DHp-CA) in complex with ADP/Mg2+

Released:
DOI: 10.2210/pdb5idj/pdb
PDB entry 5idj coloured by chain, front view.
PDB entry 5idj coloured by chain, side view.
PDB entry 5idj coloured by chain, top view.
Source organism: Caulobacter vibrioides
Primary publication:
Cyclic di-GMP mediates a histidine kinase/phosphatase switch by noncovalent domain cross-linking.
Dubey BN, Lori C, Ozaki S, Fucile G, Plaza-Menacho I, Jenal U, Schirmer T
Sci Adv 2 e1600823 (2016)
PMID: 27652341

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194928 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chain: A
Molecule details ›
Chain: A
Length: 242 amino acids
Theoretical weight: 26.54 KDa
Source organism: Caulobacter vibrioides
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X688 (Residues: 304-545; Coverage: 35%)
Gene name: cckA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P6322
Unit cell:
a: 164.856Å b: 164.856Å c: 48.001Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.242 0.239 0.289
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Cyclic di-GMP: second messenger extraordinaire.
Jenal et al. (2017)
6 more

10 mentions without citation

What method to use for protein-protein docking?
Porter et al. (2019)
9 more