PDBe 5i25

X-ray diffraction
2.85Å resolution

human recombinant coagulation FXI in complex with a peptide derived from human high molecular weight kininogen (HKP)

Released:

Function and Biology Details

Reaction catalysed:
Selective cleavage of Arg-|-Ala and Arg-|-Val bonds in factor IX to form factor IXa.
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Coagulation factor XI Chain: A
Molecule details ›
Chain: A
Length: 607 amino acids
Theoretical weight: 68.12 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P03951 (Residues: 19-625; Coverage: 100%)
Gene name: F11
Sequence domains:
Structure domains:
Kininogen-1 light chain Chain: B
Molecule details ›
Chain: B
Length: 8 amino acids
Theoretical weight: 904 Da
Source organism: Homo sapiens
Expression system: synthetic construct
UniProt:
  • Canonical: P01042 (Residues: 601-608; Coverage: 1%)
Gene names: BDK, KNG, KNG1

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P43212
Unit cell:
a: 80.59Å b: 80.59Å c: 251.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.226 0.288
Expression systems:
  • Cricetulus griseus
  • synthetic construct