› 5i0f

X-ray diffraction
1.85Å resolution

Cycloalternan-degrading enzyme from Trueperella pyogenes in complex with covalent intermediate

Released:
DOI: 10.2210/pdb5i0f/pdb
PDB entry 5i0f coloured by chain, front view.
PDB entry 5i0f coloured by chain, side view.
PDB entry 5i0f coloured by chain, top view.
Source organism: Trueperella pyogenes
Primary publication:
Transferase Versus Hydrolase: The Role of Conformational Flexibility in Reaction Specificity.
Light SH, Cahoon LA, Mahasenan KV, Lee M, Boggess B, Halavaty AS, Mobashery S, Freitag NE, Anderson WF
Structure 25 295-304 (2017)
PMID: 28089449

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165765 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Glycoside hydrolase family 31 Chain: B
Molecule details ›
Chain: B
Length: 733 amino acids
Theoretical weight: 82.33 KDa
Source organism: Trueperella pyogenes
Expression system: Escherichia coli
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC, BGC
Carbohydrate polymer
2 bound ligands:
Ligand CA 8 x CA
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 195.127Å b: 103.128Å c: 44.094Å
α: 90° β: 92.14° γ: 90°
R-values:
R R work R free
0.16 0.159 0.19
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Synthesis of novel α-glucans with potential health benefits through controlled glucose release in the human gastrointestinal tract.
Gangoiti et al. (2020)
2 more

1 mention without citation

Transferase Versus Hydrolase: The Role of Conformational Flexibility in Reaction Specificity.
Light et al. (2017)