PDBe 5hps

X-ray diffraction
2.05Å resolution

System-wide modulation of HECT E3 ligases with selective ubiquitin variant probes: WWP1 and UbV P1.1

Released:

Function and Biology Details

Reaction catalysed:
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NEDD4-like E3 ubiquitin-protein ligase WWP1 Chain: A
Molecule details ›
Chain: A
Length: 383 amino acids
Theoretical weight: 45.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H0M0 (Residues: 537-917; Coverage: 41%)
Gene name: WWP1
Ubiquitin Chain: B
Molecule details ›
Chain: B
Length: 83 amino acids
Theoretical weight: 9.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62987 (Residues: 1-73; Coverage: 57%)
Gene names: UBA52, UBCEP2

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 207.914Å b: 44.908Å c: 60.2Å
α: 90° β: 96.07° γ: 90°
R-values:
R R work R free
0.211 0.209 0.266
Expression system: Escherichia coli BL21(DE3)