PDBe 5hpl

X-ray diffraction
2.31Å resolution

System-wide modulation of HECT E3 ligases with selective ubiquitin variant probes: Rsp5 and UbV R5.4

Released:

Function and Biology Details

Reaction catalysed:
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase RSP5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 380 amino acids
Theoretical weight: 44.86 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P39940 (Residues: 430-809; Coverage: 47%)
Gene names: MDP1, NPI1, RSP5, SYGP-ORF41, YER125W
Sequence domains: HECT-domain (ubiquitin-transferase)
Ubiquitin Chains: C, D
Molecule details ›
Chains: C, D
Length: 86 amino acids
Theoretical weight: 9.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62987 (Residues: 1-77; Coverage: 60%)
Gene names: UBA52, UBCEP2
Sequence domains: Ubiquitin family

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 72.486Å b: 92.352Å c: 82.161Å
α: 90° β: 101.6° γ: 90°
R-values:
R R work R free
0.194 0.191 0.254
Expression system: Escherichia coli