PDBe 5hho

X-ray diffraction
2.95Å resolution

Crystal Structure of the JM22 TCR in complex with HLA-A*0201 in complex with M1-G4E

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A-2 alpha chain Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P01892 (Residues: 25-300; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
T cell receptor alpha constant; T cell receptor alpha variable 27 Chain: D
Molecule details ›
Chain: D
Length: 199 amino acids
Theoretical weight: 21.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P01848 (Residues: 1-89; Coverage: 64%)
  • Canonical: NEW A0A087WT01 (Residues: 20-109; Coverage: 100%)
  • nullnull
Gene names: TCRA, TRAC, TRAV27
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
T cell receptor beta constant 2; T cell receptor beta variable 19 Chain: E
Molecule details ›
Chain: E
Length: 241 amino acids
Theoretical weight: 27.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW A0A5B9 (Residues: 1-129; Coverage: 73%)
  • Canonical: NEW A0A075B6N1 (Residues: 21-114; Coverage: 100%)
  • nullnull
Gene names: TCRBC2, TRBC2, TRBV19
Sequence domains:
Structure domains: Immunoglobulins
M1-G4E, GILEFVFTL Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.04 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: C2
Unit cell:
a: 233.657Å b: 49.033Å c: 113.04Å
α: 90° β: 115.95° γ: 90°
R-values:
R R work R free
0.238 0.236 0.279
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided