PDBe 5hhm

X-ray diffraction
2.5Å resolution

Crystal Structure of the JM22 TCR in complex with HLA-A*0201 in complex with M1-F5L

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
M1-F5L, GILGLVFTL Chains: C, H
Molecule details ›
Chains: C, H
Length: 9 amino acids
Theoretical weight: 932 Da
Source organism: synthetic construct
Expression system: Not provided
T cell receptor beta variable 19; T cell receptor beta constant 2 Chains: E, J
Molecule details ›
Chains: E, J
Length: 241 amino acids
Theoretical weight: 27.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A5B9 (Residues: 1-129; Coverage: 73%)
  • Canonical: A0A075B6N1 (Residues: 21-113; Coverage: 99%)
  • nullnull
Gene names: TCRBC2, TRBC2, TRBV19
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Beta-2-microglobulin Chains: B, G
Molecule details ›
Chains: B, G
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
T cell receptor alpha variable 27; T cell receptor alpha constant Chains: D, I
Molecule details ›
Chains: D, I
Length: 200 amino acids
Theoretical weight: 21.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01848 (Residues: 1-19, 20-91; Coverage: 64%)
  • Canonical: A0A087WT01 (Residues: 20-109; Coverage: 100%)
  • nullnull
Gene names: TCRA, TRAC, TRAV27
Sequence domains:
Structure domains: Immunoglobulins
HLA class I histocompatibility antigen, A-2 alpha chain Chains: A, F
Molecule details ›
Chains: A, F
Length: 276 amino acids
Theoretical weight: 31.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01892 (Residues: 25-300; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains: MHC class I-like antigen recognition-like

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: C2
Unit cell:
a: 242.876Å b: 47.296Å c: 185.945Å
α: 90° β: 115.49° γ: 90°
R-values:
R R work R free
0.22 0.216 0.289
Expression systems:
  • Not provided
  • Escherichia coli BL21(DE3)