PDBe 5gn8

X-ray diffraction
2.8Å resolution

Structure of a 48-mer protein nanocage fabricated from its 24-mer analogue by subunit interface redesign

Released:

Function and Biology Details

Reaction catalysed:
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ferritin heavy chain Chain: A
Molecule details ›
Chain: A
Length: 176 amino acids
Theoretical weight: 20.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02794 (Residues: 2-183; Coverage: 96%)
Gene names: FTH, FTH1, FTHL6, OK/SW-cl.84, PIG15
Sequence domains: Ferritin-like domain
Structure domains: Ferritin
Ferritin heavy chain, N-terminally processed Chain: B
Molecule details ›
Chain: B
Length: 146 amino acids
Theoretical weight: 17.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02794 (Residues: 2-153; Coverage: 80%)
Gene names: FTH, FTH1, FTHL6, OK/SW-cl.84, PIG15
Structure domains: Ferritin

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: F432
Unit cell:
a: 236.12Å b: 236.12Å c: 236.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.251
Expression system: Escherichia coli