PDBe 5gi4

X-ray diffraction
2.24Å resolution

DEAD-box RNA helicase

Source organism: Escherichia coli K-12
Entry authors: Xu L, Wang L, Li F, Wu L, Shi Y

Function and Biology Details

Reaction catalysed:
ATP + H(2)O = ADP + phosphate. 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
ATP-dependent RNA helicase DeaD Chains: A, B
Molecule details ›
Chains: A, B
Length: 228 amino acids
Theoretical weight: 25.75 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
  • Canonical: P0A9P6 (Residues: 218-445; Coverage: 36%)
Gene names: JW5531, b3162, csdA, deaD, mssB, rhlD
Sequence domains: Helicase conserved C-terminal domain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 55.617Å b: 86.985Å c: 117.851Å
α: 90° β: 90° γ: 90°
R R work R free
0.227 0.225 0.268
Expression system: Escherichia coli K-12