PDBe 5fq5

X-ray diffraction
2.14Å resolution

Crystal structure of Cas9-sgRNA-DNA complex solved by native SAD phasing

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: B
Molecule details ›
Chain: B
Length: 1372 amino acids
Theoretical weight: 158.88 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
NON-TARGET DNA STRAND Chain: D
Molecule details ›
Chain: D
Length: 11 nucleotides
Theoretical weight: 3.41 KDa
Source organism: synthetic construct
Expression system: Not provided
TARGET DNA STRAND DISTAL FRAGMENT Chain: E
Molecule details ›
Chain: E
Length: 17 nucleotides
Theoretical weight: 5.22 KDa
Source organism: synthetic construct
Expression system: Not provided
TARGET DNA STRAND PROXIMAL FRAGMENT Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.3 KDa
Source organism: synthetic construct
Expression system: Not provided
SGRNA Chain: A
Molecule details ›
Chain: A
Length: 84 nucleotides
Theoretical weight: 27.12 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 177.74Å b: 67.57Å c: 188.19Å
α: 90° β: 111.31° γ: 90°
R-values:
R R work R free
0.192 0.191 0.225
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided