PDBe 5fi0

X-ray diffraction
3.28Å resolution

Crystal Structure of the P-Rex1 DH/PH tandem in complex with Rac1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 374 amino acids
Theoretical weight: 43.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8TCU6 (Residues: 38-408; Coverage: 22%)
Gene names: KIAA1415, PREX1
Sequence domains: RhoGEF domain
Structure domains:
Ras-related C3 botulinum toxin substrate 1 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 195 amino acids
Theoretical weight: 21.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63000 (Residues: 1-192; Coverage: 100%)
Gene names: MIG5, RAC1, TC25
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 81.373Å b: 107.053Å c: 323.568Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.236
Expression system: Escherichia coli BL21(DE3)