PDBe 5fd2

X-ray diffraction
2.89Å resolution

B-Raf wild-type kinase domain in complex with a purinylpyridinylamino-based inhibitor

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase B-raf Chains: A, B
Molecule details ›
Chains: A, B
Length: 300 amino acids
Theoretical weight: 34.53 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: NEW P15056 (Residues: 433-726; Coverage: 38%)
Gene names: BRAF, BRAF1, RAFB1
Sequence domains: Protein tyrosine kinase
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: C2221
Unit cell:
a: 73.79Å b: 80.088Å c: 245.253Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.213 0.278
Expression system: Trichoplusia ni