PDBe 5f8t

X-ray diffraction
1.75Å resolution

The crystal structure of human Plasma Kallikrein in complex with its peptide inhibitor pkalin-2

Released:
Entry authors: Xu M, Jiang L, Xu P, Luo Z, Andreasen P, Huang M

Function and Biology Details

Reaction catalysed:
Cleaves selectively Arg-|-Xaa and Lys-|-Xaa bonds, including Lys-|-Arg and Arg-|-Ser bonds in (human) kininogen to release bradykinin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Plasma kallikrein light chain Chain: A
Molecule details ›
Chain: A
Length: 239 amino acids
Theoretical weight: 26.91 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P03952 (Residues: 391-629; Coverage: 39%)
Gene names: KLK3, KLKB1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
CYS-PRO-LYS-ARG-PHE-M70-ALA-LEU-PHE-CYS Chain: P
Molecule details ›
Chain: P
Length: 10 amino acids
Theoretical weight: 1.16 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P65
Unit cell:
a: 90.153Å b: 90.153Å c: 57.26Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.231 0.231 0.245
Expression systems:
  • Komagataella pastoris
  • Not provided