PDBe 5f1b

X-ray diffraction
2.3Å resolution

Structural basis of Ebola virus entry: viral glycoprotein bound to its endosomal receptor Niemann-Pick C1

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
GP1 Chain: A
Molecule details ›
Chain: A
Length: 158 amino acids
Theoretical weight: 17.19 KDa
Source organism: Ebola virus - Zaire (1995)
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P87666 (Residues: 32-188; Coverage: 24%)
Gene name: GP
GP2-delta Chain: B
Molecule details ›
Chain: B
Length: 130 amino acids
Theoretical weight: 14.88 KDa
Source organism: Ebola virus - Zaire (1995)
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P87666 (Residues: 509-632; Coverage: 19%)
Gene name: GP
NPC intracellular cholesterol transporter 1 Chain: C
Molecule details ›
Chain: C
Length: 256 amino acids
Theoretical weight: 29.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15118 (Residues: 374-620; Coverage: 20%)
Gene name: NPC1

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P63
Unit cell:
a: 107.261Å b: 107.261Å c: 93.777Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.181 0.23
Expression systems:
  • Trichoplusia ni
  • Escherichia coli