PDBe 5f0l

X-ray diffraction
3.2Å resolution

Structure of retromer VPS26-VPS35 subunits bound to SNX3 and DMT1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Sorting nexin-3 Chain: C
Molecule details ›
Chain: C
Length: 167 amino acids
Theoretical weight: 19.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O60493 (Residues: 1-162; Coverage: 100%)
Gene name: SNX3
Sequence domains: PX domain
Vacuolar protein sorting-associated protein 35 Chain: A
Molecule details ›
Chain: A
Length: 462 amino acids
Theoretical weight: 53.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96QK1 (Residues: 14-470; Coverage: 57%)
Gene names: MEM3, TCCCTA00141, VPS35
Vacuolar protein sorting-associated protein 26A Chain: B
Molecule details ›
Chain: B
Length: 317 amino acids
Theoretical weight: 37.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75436 (Residues: 1-317; Coverage: 97%)
Gene names: VPS26, VPS26A
Sequence domains: Vacuolar protein sorting-associated protein 26
Structure domains: Immunoglobulin-like
Natural resistance-associated macrophage protein 2 Chain: D
Molecule details ›
Chain: D
Length: 24 amino acids
Theoretical weight: 2.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49281 (Residues: 545-568; Coverage: 4%)
Gene names: DCT1, DMT1, NRAMP2, OK/SW-cl.20, SLC11A2

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 371.37Å b: 75.21Å c: 57.45Å
α: 90° β: 98.56° γ: 90°
R-values:
R R work R free
0.214 0.212 0.259
Expression system: Escherichia coli BL21(DE3)